FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345368
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC16500.4777512682124574Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT13860.4013110652984642TruSeq Adapter, Index 2 (100% over 39bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA12650.3662759722962174TruSeq Adapter, Index 2 (100% over 40bp)
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT12290.35585230826249103TruSeq Adapter, Index 2 (100% over 37bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA9450.2736211808853165TruSeq Adapter, Index 2 (100% over 36bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG8320.24090245766834215TruSeq Adapter, Index 2 (100% over 38bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7940.2298997011882977Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4200.12160941372680735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)