FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005716509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346184
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC18100.5228433434243062Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT14810.4278071776858549TruSeq Adapter, Index 2 (100% over 39bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA14090.4070089894391422TruSeq Adapter, Index 2 (100% over 40bp)
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT12270.3544357913710628TruSeq Adapter, Index 2 (100% over 37bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA9450.2729762207381046TruSeq Adapter, Index 2 (100% over 36bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG8790.2539112148452846TruSeq Adapter, Index 2 (100% over 38bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7890.2279134795368937Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4170.12045617359554457Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3540.10225775887967092Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)