FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320128
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA14600.4560675729708117TruSeq Adapter, Index 2 (97% over 36bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT8370.2614579168332667Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC6970.21772540983606556TruSeq Adapter, Index 4 (100% over 38bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA6910.21585115953618556TruSeq Adapter, Index 4 (100% over 39bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6320.19742103158736507Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC5520.17243102758896442TruSeq Adapter, Index 4 (100% over 37bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG5310.16587115153938425TruSeq Adapter, Index 2 (97% over 35bp)
GAGGCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA3480.1087065173930428TruSeq Adapter, Index 4 (100% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)