FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318497
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA13480.42323789549038143TruSeq Adapter, Index 2 (97% over 36bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT7940.24929591173543236Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA6660.20910715014584125TruSeq Adapter, Index 4 (100% over 39bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC6480.20345560554730502TruSeq Adapter, Index 4 (100% over 38bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5830.18304725005259076Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC5430.17048826205584353TruSeq Adapter, Index 4 (100% over 37bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG4680.1469401595619425TruSeq Adapter, Index 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)