FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318124
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA14490.45548276772579244TruSeq Adapter, Index 2 (97% over 36bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT8800.27662169468509135Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC7400.23261369780337224TruSeq Adapter, Index 4 (100% over 38bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA6720.21123838503225156TruSeq Adapter, Index 4 (100% over 39bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5770.1813758157196565Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC5520.17351724484792094TruSeq Adapter, Index 4 (100% over 37bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG4920.15465667475575562TruSeq Adapter, Index 2 (97% over 35bp)
GAGGCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA4020.12636581961750765TruSeq Adapter, Index 4 (100% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)