FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335750
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC12060.3591958302308265Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA10600.31571109456440805TruSeq Adapter, Index 2 (100% over 36bp)
TCCATTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT9580.285331347728965TruSeq Adapter, Index 2 (100% over 37bp)
TCCATTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA7200.2144452717795979TruSeq Adapter, Index 2 (100% over 40bp)
TCCATTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6970.20759493670886076Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4290.12777364110201042Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT4010.11943410275502607TruSeq Adapter, Index 2 (100% over 39bp)
TCCATTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG3820.11377513030528666TruSeq Adapter, Index 2 (100% over 38bp)
TCCATTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3510.10454206999255398Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)