FastQCFastQC Report
Wed 8 May 2024
EGAF00005716623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252435
Total Bases16.3 Mbp
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTACTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4740.1877711093944976Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA3670.14538396022738528TruSeq Adapter, Index 4 (100% over 36bp)
CATTACTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG3040.12042704062431914TruSeq Adapter, Index 4 (100% over 35bp)
CATTACTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC2930.11606948323330758TruSeq Adapter, Index 4 (100% over 37bp)
CATTACTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2570.1018083863172698Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)