FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252435
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTACTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4730.18737496781349652Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA3650.14459167706538317TruSeq Adapter, Index 2 (97% over 36bp)
CATTACTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG3030.12003089904331808TruSeq Adapter, Index 2 (97% over 35bp)
CATTACTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC2910.11527720007130549TruSeq Adapter, Index 4 (100% over 37bp)
CATTACTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2560.10141224473626874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)