FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321253
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC14320.4457545921750147Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT7980.24840234955004312TruSeq Adapter, Index 2 (100% over 43bp)
CTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC6830.21260501847453567TruSeq Adapter, Index 2 (100% over 42bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT6550.20388914656049906TruSeq Adapter, Index 2 (100% over 37bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG5600.174317438280732TruSeq Adapter, Index 2 (100% over 35bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA5040.1568856944526588TruSeq Adapter, Index 2 (100% over 36bp)
CTGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT4900.15252775849564051TruSeq Adapter, Index 2 (100% over 41bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA4740.14754726025904816TruSeq Adapter, Index 2 (100% over 40bp)
GGATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT4060.1263801427535307TruSeq Adapter, Index 2 (100% over 41bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3780.11766427083949411Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT3600.11206121032332772TruSeq Adapter, Index 2 (100% over 39bp)
GGATGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG3220.10023252701142091TruSeq Adapter, Index 2 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)