FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318789
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC13420.42096810115781913Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT9050.2838868342383206TruSeq Adapter, Index 2 (100% over 43bp)
CTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC6770.21236617323684318TruSeq Adapter, Index 2 (100% over 42bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT5740.18005640094231606TruSeq Adapter, Index 2 (100% over 37bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA5240.16437204545953593TruSeq Adapter, Index 2 (100% over 40bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA5120.16060780014366868TruSeq Adapter, Index 2 (100% over 36bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG4830.1515108739636562TruSeq Adapter, Index 2 (100% over 35bp)
CTGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT4420.13864970246777647TruSeq Adapter, Index 2 (100% over 41bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4080.127984340739486Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT3910.12265165987534075TruSeq Adapter, Index 2 (100% over 41bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT3470.10884942705049422TruSeq Adapter, Index 2 (100% over 39bp)
GGATGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG3440.1079083657215274TruSeq Adapter, Index 2 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)