FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324425
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC14710.45341758495800255Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT9260.28542806503814444TruSeq Adapter, Index 2 (100% over 43bp)
CTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC6940.21391692995299375TruSeq Adapter, Index 2 (100% over 42bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT5970.1840178777837713TruSeq Adapter, Index 2 (100% over 37bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA5560.1713801340833783TruSeq Adapter, Index 2 (100% over 36bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA5190.15997534098790167TruSeq Adapter, Index 2 (100% over 40bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG5010.15442706326577793TruSeq Adapter, Index 2 (100% over 35bp)
GGATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT4450.13716575479694845TruSeq Adapter, Index 2 (100% over 41bp)
CTGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT4410.13593280419203208TruSeq Adapter, Index 2 (100% over 41bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT3610.11127379209370425TruSeq Adapter, Index 2 (100% over 39bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3450.1063419896740387Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG3420.1054172767203514TruSeq Adapter, Index 2 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)