FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005716637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319832
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC14230.44492108356887367Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT10670.3336126466394857TruSeq Adapter, Index 2 (100% over 43bp)
CTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC7140.22324220215613194TruSeq Adapter, Index 2 (100% over 42bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT6380.1994797268565997TruSeq Adapter, Index 2 (100% over 37bp)
GGATGGCCTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG5900.18447184772005304TruSeq Adapter, Index 2 (100% over 35bp)
GGATGGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA5420.16946396858350635TruSeq Adapter, Index 2 (100% over 40bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA4880.15258010455489132TruSeq Adapter, Index 2 (100% over 36bp)
CTGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT4870.15226744040621326TruSeq Adapter, Index 2 (100% over 41bp)
GGATGGCCTGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4440.13882288201305684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT4200.1313189424447835TruSeq Adapter, Index 2 (100% over 41bp)
GGATGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT3580.11193376522674403TruSeq Adapter, Index 2 (100% over 39bp)
GGATGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG3500.10943245203731959TruSeq Adapter, Index 2 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)