FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415036
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC42291.0189477539297795Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC15420.3715340356017309Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG14950.3602097167474629TruSeq Adapter, Index 2 (100% over 35bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG14770.35587274356923254TruSeq Adapter, Index 2 (100% over 38bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT12570.3028652936130842TruSeq Adapter, Index 2 (100% over 37bp)
CATGGCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT10370.24985784365693578TruSeq Adapter, Index 2 (100% over 39bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA10200.2457618134330516TruSeq Adapter, Index 2 (100% over 36bp)
CATGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA5970.14384294374463902TruSeq Adapter, Index 2 (100% over 40bp)
CATGGCTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5230.12601316512302546Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)