FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410137
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC43921.0708616876799704Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG15550.37914160390308604TruSeq Adapter, Index 2 (100% over 35bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14900.36329324103896987Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG14780.36036738943328694TruSeq Adapter, Index 2 (100% over 38bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT13420.3272077379022132TruSeq Adapter, Index 2 (100% over 37bp)
CATGGCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT11510.2806379331784258TruSeq Adapter, Index 2 (100% over 39bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA10840.2643019283800291TruSeq Adapter, Index 2 (100% over 36bp)
CATGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA5870.14312290771132571TruSeq Adapter, Index 2 (100% over 40bp)
CATGGCTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5090.12410487227438637Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph