FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422155
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAACTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC17560.41596096220582485Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG7980.18903009558100697TruSeq Adapter, Index 2 (100% over 35bp)
TAGAACTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT7200.17055346969714916TruSeq Adapter, Index 2 (100% over 37bp)
TAGAACTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6580.15586692091767243Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGAACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT5180.12270374625433786TruSeq Adapter, Index 2 (100% over 39bp)
TAGAACTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA5180.12270374625433786TruSeq Adapter, Index 2 (100% over 36bp)
TAGAACTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG4530.10730655801778968TruSeq Adapter, Index 2 (100% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)