FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267463
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAACTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC7400.2766737829157678TruSeq Adapter, Index 4 (100% over 37bp)
GTAACTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA7210.26956999659766023TruSeq Adapter, Index 2 (97% over 36bp)
GTAACTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT4720.17647300748140865Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTAACTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC4110.15366611456537912TruSeq Adapter, Index 4 (100% over 38bp)
GTAACTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3710.1387107749483106Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)