FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266549
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAACTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC7510.28174932188828317TruSeq Adapter, Index 4 (100% over 37bp)
GTAACTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA7410.27799766647033003TruSeq Adapter, Index 2 (97% over 36bp)
GTAACTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT4820.18082979114534287Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTAACTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC4470.16769899718250678TruSeq Adapter, Index 4 (100% over 38bp)
GTAACTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3650.13693542275529078Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTAACTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA2950.11067383482961857TruSeq Adapter, Index 4 (100% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)