FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385782
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC14180.367565101534027Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA7160.1855970470369276TruSeq Adapter, Index 2 (100% over 36bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6500.16848893934916612Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG5770.14956633539149053TruSeq Adapter, Index 2 (100% over 38bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT5160.13375429646795342TruSeq Adapter, Index 2 (100% over 39bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT4150.10757370743062145TruSeq Adapter, Index 2 (100% over 37bp)
TTACCTTGACTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG3970.10290785987941377TruSeq Adapter, Index 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)