FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388551
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC15110.3888807389506139Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA7390.1901938226899429TruSeq Adapter, Index 2 (100% over 36bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6800.17500920085136829Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG6090.1567361813507107TruSeq Adapter, Index 2 (100% over 38bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT5650.14541205658973982TruSeq Adapter, Index 2 (100% over 39bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT4660.11993277587755533TruSeq Adapter, Index 2 (100% over 37bp)
TTACCTTGACTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG4300.11066758289130642TruSeq Adapter, Index 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)