FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306965
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATACTTCTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT17310.5639079373869985Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCATACTTCTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG5330.17363543074943397TruSeq Adapter, Index 2 (97% over 35bp)
TCATACTTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC4710.15343768833580376TruSeq Adapter, Index 4 (100% over 37bp)
TCATACTTCTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA4420.14399035720684766TruSeq Adapter, Index 2 (97% over 36bp)
TCATACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA3130.10196602218493964TruSeq Adapter, Index 4 (100% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)