FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598730
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA32120.5364688590850634TruSeq Adapter, Index 2 (100% over 36bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC27950.46682143871194026TruSeq Adapter, Index 2 (100% over 42bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC20320.3393850316503265Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT13680.2284836236701017TruSeq Adapter, Index 2 (100% over 37bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG12300.205434837071802TruSeq Adapter, Index 2 (100% over 35bp)
GACGACCAGATTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6490.10839610508910527Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT6230.10405358007783141TruSeq Adapter, Index 2 (100% over 43bp)
GACGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT6090.10171529737945317TruSeq Adapter, Index 2 (100% over 41bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)