FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590075
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA32230.5462017540143203TruSeq Adapter, Index 2 (100% over 36bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC30030.5089183578358684TruSeq Adapter, Index 2 (100% over 42bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC20660.3501249841121891Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG13980.23691903571579884TruSeq Adapter, Index 2 (100% over 35bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT13960.23658009575054018TruSeq Adapter, Index 2 (100% over 37bp)
GACGACCAGATTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6740.11422276829216625Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT6630.11235859848324366TruSeq Adapter, Index 2 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6160.10439350929966529TruSeq Adapter, Index 2 (100% over 63bp)
GACGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT6080.1030377494386307TruSeq Adapter, Index 2 (100% over 41bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)