FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005716741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005716741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296036
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAATTCTATTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT10050.3394857382210272Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAATTCTATTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA6870.23206637030631408TruSeq Adapter, Index 2 (97% over 36bp)
TCAATTCTATTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG4700.15876447459092813TruSeq Adapter, Index 2 (97% over 35bp)
TCAATTCTATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC4620.15606210055533787TruSeq Adapter, Index 4 (100% over 37bp)
TCAATTCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA3500.11822886405707415TruSeq Adapter, Index 4 (100% over 40bp)
TCAATTCTATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC3190.10775716466916185TruSeq Adapter, Index 4 (100% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)