FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9886399
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT1552.5495727E-517.54830211
TACACTG41600.014.139715
TACGCGA1750.001268246713.60000161
CGACCGT1750.001268295113.59993444
CTACACT30750.013.3791334
TCGTCGA1800.001568327413.22222441
TCGGACG1550.00893830313.16122610
TATACCG5200.013.07718855
CTAGCAC20650.013.007533
TATACTG43500.012.8968145
TACACCG7450.012.7787845
GTGTAGG29050.012.4064491
GTATAGG18200.012.14310551
GTATAGA32050.011.7755921
TATACGC4652.5993359E-911.6991635
CCTATAC16900.011.6688783
TAGCACT40300.011.6429174
GTACTAG19700.011.5636871
ATACTGT66350.011.3762676
CTATACT30500.011.3707244
Produced by FastQC (version 0.11.5)