FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9109707
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC2652.7739588E-916.679553
TATACGC4850.016.1240082
CGTAACG1300.00278165115.6922539
ATCGCGC1400.00455493714.5713778
TACACTG39450.014.0484215
CTAGACT19350.013.5299644
TATACTG43050.013.4264955
CTACACT26900.013.3980154
TCTAGAC19950.012.9526193
GTCTAGA21500.012.6513941
ATACGCC5157.2759576E-1212.543923
CCTATAC15300.012.4446733
AACGACG2751.8983568E-512.36372761
TGCGCGG5351.2732926E-1112.07472210
TAGACTG26800.011.7987235
GTATACG9050.011.6466221
CTGCGCG7300.011.6437939
GTCTAAC14750.011.5256351
GTATTAC36100.011.3021461
CTATACT28400.011.2537284
Produced by FastQC (version 0.11.5)