FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9109707
Sequences flagged as poor quality0
Sequence length74
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19100.015.486081
TACTCCG6650.015.3371855
CGTTGCG2001.31341E-515.2998560
TACACCG6800.014.9988645
TATACTG41700.014.7566785
CTACACT28150.014.7342324
ACGCGAT1450.005751949714.06790217
TACACTG38450.013.6166555
TCGTTCG1750.001268958313.59897166
CTATACT30500.013.3760384
TCTAGAC20650.013.006283
CTGCGCG6600.012.8778149
GTCGCGT1850.001924895112.8671458
CGCGTAT2154.1405886E-412.65020612
TACGCCG1950.002853687912.20963450
GTATAGG17600.012.1698021
CCTATAC14800.011.9450433
GTCTAGA21650.011.7776591
GTAATAC30250.011.5759843
GCTATAC18450.011.424613
Produced by FastQC (version 0.11.5)