FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7877959
Sequences flagged as poor quality0
Sequence length74
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90470.11483938923774546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA36950.017.38941468
CGACGAA26550.017.15844268
GCGTTAC2303.0032643E-614.7811991
GCCGACG49650.013.14678367
ATAGCGT3954.0092345E-812.0494858
TCTGACG196550.011.39855868
ATCTGAC246950.011.30242568
CGTACGG2150.005937300611.06871264
CTGCCGA78150.011.0494968
TGCCGAC63250.011.01871368
TTAGTCG2200.00703417810.81921159
GTATTAG9850.010.6994881
TACACCG13000.010.4605425
CATCTGA317700.010.41197668
CTACACT19350.010.3659374
TACACTG27950.010.3389075
CTGACGC160400.010.08881568
GTATTAA17750.09.7680841
CTAACCG3855.3665884E-59.713365
GTATTGG23350.09.6093631
Produced by FastQC (version 0.11.5)