FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9200781
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96530.10491500667171624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT850.0044877919.9984942
TAGACCG3607.239578E-1014.1655965
TACACCG6300.014.0306855
CGGAACG1951.8057048E-413.95388255
ATACGCC4559.094947E-1213.44953253
GTATTAG20800.013.23961
CGCGCGT1800.001569207813.22122316
TACACTG40600.012.89557655
CTACACT28300.012.8541524
TATACTG43950.012.8408955
GTACTAG18700.012.3629041
GTGTAGG32200.012.3533041
CGATGCG2501.0715533E-412.23907568
GTATAGA28950.012.2134381
ACGCGAA2256.072024E-412.08797541
AGACCGT5653.6379788E-1212.0344886
ATTAGAC25100.011.9194173
CTATACT32000.011.89914
CCTATAC14500.011.7232513
GTCTAGT14050.011.3729921
Produced by FastQC (version 0.11.5)