FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9850620
Sequences flagged as poor quality0
Sequence length74
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT30350.014.0022654
TATACTG43800.013.9715765
TACACTG42100.013.8089615
GTATTAG20600.013.0380751
GTGTAGG32600.012.8274731
TACACCG8250.012.7748175
ACTATAC21850.012.6031923
CGATGCG3358.7168337E-712.17820868
CTATACT29550.012.080334
CTAGACT20100.012.0090674
TCTACAC28300.011.6528533
GTATACG9350.011.6356861
GTATAGA29700.011.4471451
TACTCCG6850.011.4152195
TCTAGAC22700.011.3824233
GCGGTTA5452.3101165E-1011.22853224
GTAACAC36300.011.1451863
GTCCTAC20200.011.1082631
GTATAAG32800.011.09081651
CGGTTAC5603.7107384E-1010.92776825
Produced by FastQC (version 0.11.5)