FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8816167
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT1351.7647522E-417.62992919
TACACTG37300.015.22269255
TATACGC4400.014.6819862
GTATAGG16250.014.646321
TACGCGA1400.004554314.57167621
CTAGGAC18050.014.3159513
CTACACT25700.014.1558034
CACGCGT1700.00101880514.0001584
GTATACG7850.013.860031
GCGTCGT2002.2452808E-413.59992445
CCTATAC15400.013.4676863
TCTAGAC20200.013.2971813
GTATTAG17550.013.1739381
CGATGCG2356.002062E-513.02127668
TATACTG41300.013.0074115
CTATACT27700.012.8882324
GTAACGC2651.297318E-512.8303353
TAGGACT28450.012.7874924
GTATTAC30800.012.58455851
CTAATAC22450.012.26743
Produced by FastQC (version 0.11.5)