FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8816167
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG659.436978E-426.15161120
CTAGACG2401.4782927E-816.9985474
CGCGAAC1402.3319924E-416.99854718
CGCGTAT1951.0340329E-515.69096712
TACTCCG6350.015.5262315
TACACTG38350.014.6271725
TACACCG6050.014.6103215
CTACACT27300.013.9475244
CCTATAC15000.013.8254853
TATACTG39550.013.1518475
CTATACT29800.013.0055994
GTATTAG16900.012.8747121
GTACTAG19100.012.8157311
GTATACG8200.012.4380311
CTAGCAC18550.012.2792733
ACACCGC7200.012.2767286
GTACTAT24700.012.2500341
CTAATAC21700.012.0634843
TATACTA29600.012.0597815
GTATAGG18350.012.0426351
Produced by FastQC (version 0.11.5)