FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9439581
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAC1259.7865806E-519.04015561
CGACGTA2102.0832022E-514.57131827
GTATTAG20400.014.0003391
GTATAGG17500.013.2117481
TACACTG43000.013.204975
TCTAGAC23200.012.7503083
TAGACTG27950.012.6514695
TGCGCGG6200.012.61280710
CACGCGT1900.002355190712.52621910
CTAATAC26350.012.3873963
GTATACG8550.012.3277831
CTACACT28350.012.1131684
CCGCACT4803.3469405E-1012.0419574
TAGGACT31450.011.8921794
GTAACGC3155.8262067E-611.8733023
GCGTTAC3451.2419514E-611.8263731
GTACTCG4602.1918822E-911.8263731
GTATTAC31150.011.7884071
GCGTTAG2601.5431311E-411.7695151
CGACTAT3803.149271E-711.6314910
Produced by FastQC (version 0.11.5)