FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9439581
Sequences flagged as poor quality0
Sequence length74
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG39450.014.9950515
GTCGTAC2507.1200884E-613.5991761
TATACGC5050.013.4643162
CTACACT29150.013.4123534
TACACCG7750.013.1602845
TATACTG41850.012.6728655
TAGAGCG4651.9645086E-1012.4291564
TAGCACT36000.012.1824014
GTATACG8100.012.1721021
CTAGCAC19550.011.9990823
TCGGCGC2000.003460711311.89915237
CTAATAC21600.011.8046523
GTAATAC27950.011.7987393
TATACCG4904.7111826E-1011.7950165
CTATACT30000.011.7857654
TAATACT48150.011.65024
CGCTAGC2358.747506E-411.57358334
GTGTAGG32050.011.5624821
GTATTAG19450.011.5365761
CGATGCG3551.754499E-611.49207868
Produced by FastQC (version 0.11.5)