FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8807663
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG5500.014.8362189
TGCGCGG3203.346213E-813.81236410
GTATTAG17000.013.80011
TACACTG40050.013.7529085
ACGAGCG1750.001268214113.6000235
CGCGTAT1750.001268322713.59986612
GTATAGG15850.013.299781
GCGGTTA4154.311005E-1013.10845324
TACTCCG6250.013.0560945
TATACTG42450.012.4947895
CTACACT30100.012.4253394
TCTATAC24700.012.1134473
CGACTAT3155.828286E-611.87289910
TAGGACT29800.011.8658574
TAGGCTG26600.011.7594825
TCGCCGA2050.004159857511.60977433
CGGTTAC5057.767085E-1011.44549725
GTATTAC30650.011.4258581
CTACCCT26550.011.3974464
TCGTCCG2100.004979265411.33335154
Produced by FastQC (version 0.11.5)