FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7756734
Sequences flagged as poor quality0
Sequence length74
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA28900.017.52779868
CCGACGA40950.016.52106568
CTAATCG1550.00894322913.1600784
GCCGACG53750.013.15599667
CGGCTAT2256.0601823E-412.09073659
GCGTTAC3451.2434066E-611.82515051
TCTGACG201000.011.2477268
TATACCG3351.1568598E-511.163155
TATACTG20450.010.805855
ATCTGAC251650.010.61853268
TGCCGAC67900.010.56456468
GTATTAG8500.010.3991751
GTATTAC14100.010.3679721
TACCGTA3056.7640917E-410.0318627
GTATTAA17200.09.8829371
GTACTAG8700.09.76934051
CTGACGC164650.09.66324668
CTATACT15350.09.5235524
TACACCG13250.09.4934655
ACTATAC10800.09.4435743
Produced by FastQC (version 0.11.5)