FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8383404
Sequences flagged as poor quality0
Sequence length74
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88670.10576849213040432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA28100.015.7283668
CGACGAC25600.014.20987568
TAGACCG3607.239578E-1014.16560655
GCCGACG40050.013.242455568
CGTAACG1550.00894228313.16030617
ATCTGAC205400.012.38076868
TCTGACG155300.012.19354968
TGCCGAC51700.011.83663868
GTATAGG11050.011.0762271
GTATTAG13700.010.9190221
GTCTAGA14550.010.7484651
GTACTAA17350.010.5814681
GCGTAAA4552.835277E-710.460881
CTACACT23100.010.4494344
CTATACT19600.010.4073854
ATTAGAC14700.010.4073843
CTGCCGA63900.010.37481168
CGACTAT3301.21819176E-410.30225910
CATCTGA277050.010.28329568
CTGACGC122400.010.11035468
Produced by FastQC (version 0.11.5)