FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910955
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCT3203.3933247E-822.6539924
TATCGTG1700.00309560221.3214045
CGTACCA1750.00366204620.7122234
CTAGTAC6052.0008883E-1117.9734173
GTATACG3652.920513E-617.9194741
GTCTAGA7600.017.2121261
CGTACAG2550.001592772217.0571255
GTGTAGG11950.017.0280821
GTGCGTA2600.00178381216.770791
GTATAAG8300.016.6360841
CCTATAC6154.802132E-1016.502423
CCGATCA2650.002060057816.413469
CTACGAC2650.002060057816.413463
CTATACT9400.016.1951944
CGTATCC2700.002333984516.1095053
CTTACGC2850.003294223515.2980612
GTACTAG5253.5465746E-715.2268111
GTATAGC6301.132139E-814.9961021
ATAATAC15200.014.7847123
GGTCTAC6952.6138878E-914.639251
Produced by FastQC (version 0.11.5)