FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17834604
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA237770.13331947263869723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA12850.014.6674674
GTATAGG31850.014.1518521
GTATTAG46100.014.0352481
CTATACT58400.013.7782814
TCTAGAC52300.013.5834253
CTAGACT42550.012.9478864
GTATTGG78800.012.4548521
GTGCTAG61000.012.3946581
CTACACT64600.012.3436924
GTGTAGG89050.012.3274731
TAACACG25400.012.2721214
TTATACT95300.012.0184684
TACACCG33800.012.0105675
GTACTAG35800.011.9811921
GTGTTAC80000.011.5410361
TCTATAC61350.011.4615153
GTACTAA56600.011.4315371
GTCTAGA48600.011.3686361
GTGTTAA86500.011.3461551
TACACTG106300.011.3205375
Produced by FastQC (version 0.11.5)