FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537919
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC1800.004309136420.1380583
TACTCCG2556.9628004E-519.901145
CCGTATA1950.006843818418.588978145
TACACGG2359.2056947E-418.5098745
CTACGCT2000.00791984718.1242524
AGACCCG3201.9297142E-518.1242525
ACGGTCC3052.743852E-416.6386598
GTATAGA7900.016.5269031
TATACAG12800.016.4251045
GGTTAGG5753.4651748E-916.3991581
CAAGACG2800.002973202815.5350744
TTAGACC7503.092282E-1115.4660274
GCGGTAG2850.00334523615.2625283
GGTTATC3951.1702519E-414.6829392
CCTACAC9400.014.653653
CTATACC4953.299967E-614.645864
GAGTTAG5506.1339597E-714.5069481
TATACTG11200.014.2404845
GATCACG4201.962588E-413.8161411
CTACGAA3150.006502165513.8089559
Produced by FastQC (version 0.11.5)