FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397106
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1850.00505186219.5926065
TAGCACG3305.002937E-415.3769064
TTAGGGT8107.2759576E-1215.2141864
CCTATAC4351.584808E-514.9981643
CGTATCG13050.014.998163145
CTACCCT15250.014.73594
CTACACT13650.014.3389044
TAAGGGA11800.014.1296264
GTATTAG5759.906071E-713.90471
CTATACC7457.203198E-913.6224944
TGGACCG8101.6152626E-913.4245625
AGAACCG11450.013.2955935
GACCGCT6403.3975703E-612.4596717
GTATATG14700.012.3611681
GTATAAC7651.4920988E-712.3187543
TAGGGGT14200.012.2520214
GTCGTAG4755.4579775E-412.241461
CTATACT7701.6241393E-712.2387614
GTATAGA8956.866685E-912.1816211
GTGTAGG20300.012.1736191
Produced by FastQC (version 0.11.5)