FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16695118
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT70000.013.0483694
GTATTAG36550.012.7296081
GTACTAG29000.012.5341371
TAGACCG9952.0918378E-1012.3857215
TACACCG43000.011.8011685
CTACACT58950.011.55924
GTATTAA71750.011.5506221
TTATACT69250.011.5148064
GTGTTAA68250.011.5038591
GTCTTAA70150.011.5031761
GTATATA82350.011.3880281
GCTTTAT95600.011.2545011
GTTTATA83150.011.1910321
TTAGACG11051.195076E-911.1524524
GGTTTAC44450.011.1214031
GTATTTA110600.011.0427341
GTGTTAT65300.010.9102661
GTACTAA50000.010.9046991
GTCTTAC55200.010.7993391
ATAATAC68050.010.7591263
Produced by FastQC (version 0.11.5)