FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779649
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT1800.004235306820.1984791
GTATAGC5503.6379788E-1219.8312361
TATACTG12500.018.5584325
TCGACTA2359.209143E-418.5090759
GTATTAG7600.018.178631
TATACGT3602.6218313E-618.122964
GTTACGC2050.00913504217.6809373
TCGTACA2901.8278524E-417.5478272
ATATACG2500.001395025217.398043
ATCTACG3403.166768E-517.10931
CTACACT9750.016.3571324
TTGTCCG2700.002333664316.1097499
CAGCGCG3706.704262E-515.6743519
CTACGCC3254.4536707E-415.6136273
GTCTAGT4251.2348413E-515.3983691
TAAGGGT8055.456968E-1215.3088364
TCGCACC2850.003347115.2614394
GTACTAT8351.0913936E-1114.8041541
TATACAG15250.014.736465
CCATGCG4451.9632911E-514.6616839
Produced by FastQC (version 0.11.5)