FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779649
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC4659.094947E-1221.8272533
TACACCG4502.9976945E-919.332715
GTACGGA2050.00911309917.688321
CTAGTAC5351.3387762E-917.6162573
GTACTAG5553.9897714E-815.6804581
ACCGGGC7001.8007995E-1015.5352158
CTATACC6251.0519216E-815.07951454
GTAGGGC7505.0749804E-1014.4995353
CCGCGCA3000.004704953614.4995349
GTTATAC7002.9922376E-914.4995343
TAAGGGT8101.05501385E-1014.3205264
CTAGCCT10150.014.28525454
TACCCCG3050.00525050214.2618365
ACTGTGC18950.014.1552188
TATGAAC11800.014.1309023
TTATACT16550.014.0176754
TAATAGT12450.013.9754544
ACTATAG9355.456968E-1213.956773
CCTACAC11150.013.6542693
GGACTTA10101.8189894E-1213.6381756
Produced by FastQC (version 0.11.5)