FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20028586
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC60100.014.5153581
GTGTTAA84150.013.3905531
GTATTAG42700.012.7689251
CAATACT82050.012.4574194
GTATTAT82650.012.1382811
GTCTAGA52550.011.8972521
GTACTAG35050.011.8224581
GTACTAT47500.011.63163951
GTGTTAC84100.011.5832311
GTATTAA80450.011.29548551
CTATACT57350.011.1233814
TAATACT82850.011.1121664
CCTATCG11752.8012437E-1011.1052039
TCTAGAC54550.011.0298823
TACACTG115350.010.9979655
GTATAAA109850.010.9857311
TTGTACT127800.010.9474754
GTGTATA80050.010.8070351
TACACCG51700.010.6565085
GTGTAGG108900.010.4807591
Produced by FastQC (version 0.11.5)