FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19057210
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA214290.11244563081374451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG13650.015.93255
TACACCG40400.013.6372735
GTGTACG28600.013.216031
CTAGACT46950.013.1241354
CTTACGA20650.011.9358739
GTCTTAC68100.011.8478511
TTATACT99600.011.6452274
ATTATAC96900.011.59565453
TAATACT101950.011.5901154
GTCTAGA53500.011.5485881
GTATTAA104700.011.524581
TCTATAC64500.011.46377853
CTACACT72750.011.35954
GTATAGG38700.011.2694831
GTACTAG35250.011.1352071
GGCTATA45700.011.1338541
GTACGAT9852.8354407E-811.0692631
GTGCTAG66450.011.0481641
TTAGACG13803.6379788E-1211.0313224
TCTAGAC52600.011.025333
Produced by FastQC (version 0.11.5)