FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19057210
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG11000.019.7719695
TACGTCG5102.6275302E-715.6366547
CTATACT62350.015.2320934
GTCTTAG51700.015.0117421
TACACCG36000.013.6939175
GTATTAG46750.013.4981891
TATGCCG13150.013.2314315
CTACACT69650.012.6988224
GTATTAC71600.012.5616451
GTCTTAC70050.012.4254171
GTGCTAG62400.012.0888951
GTACTAG37700.011.9285651
GTATAGG45000.011.9277111
TCTATAC70700.011.8951863
TATACTG99750.011.84666255
GTATTAT96200.011.7621691
TATACGC15550.011.65580752
TAACCGG131850.011.547072145
TTACACT95600.011.3751754
GTATTAA95000.011.2999351
Produced by FastQC (version 0.11.5)