FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15223009
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC45900.015.4868681
GTGTTAC64350.012.399221
GTGTTAA66250.011.9341311
GTACTAG23450.011.7541431
CTATACT42400.011.6269274
GTCTTAC48550.011.5040741
TAATACT70800.011.4685014
CTTATCG9551.8673745E-811.3871623
ATACCGC11501.9281288E-1011.3474056
CCTATAC27700.011.2542443
GTATTAA65100.011.2535771
TTACCCG15500.011.225394
GTCTTAT51700.011.2240511
TATACTG62950.011.171155
GTATTAT63050.011.0442531
TAACGCA9251.4685065E-710.9725664
GTCTAAT35750.010.95641
ATATACG14055.456968E-1210.83604053
GTATTAG30450.010.71952151
TTATACT65000.010.7072964
Produced by FastQC (version 0.11.5)