FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19457783
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA230800.11861577446927023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG36800.014.0252241
GTACTAA58900.013.822991
CTACACT72100.013.4728554
GTATAGG42300.013.404591
GTGCTAG68300.012.559161
TACACCG31450.012.4470525
CTATACT65750.012.3484474
TATACTG106550.012.2465135
GTCTAGA57700.012.22067451
TTATACT105500.012.0934524
GTATAAG76950.012.0920691
CTAGACT49850.011.9244614
GTATAGA69900.011.7516961
TAATACT106400.011.65054
TACACTG115250.011.13334755
GTCTAGT40550.011.114761
GTGTAGG90700.011.0604061
GCGTTAC9301.5239675E-710.94321
CGTACGA4650.006468745410.9420162
GTATTAA110600.010.910691
Produced by FastQC (version 0.11.5)