FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386112
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT6950.020.8604644
TCGATAT1850.005050769519.592639
GGTATAG3602.5590216E-618.1684061
CCGCACT2000.00792215818.1231829
GTCCTAG5451.6570993E-917.3349911
TCTACAC7900.016.5167373
CCTATAC3956.3464377E-616.5167373
TACACTG11350.015.9675635
CTACACG2750.00263815115.8160254
GTACTAG4159.903419E-615.7605441
CCCTACA9550.015.2185052
TACCCTG12650.014.8996915
CGGTGAA3457.006257E-414.708679
CTATACC4502.1821077E-514.4980244
CCTACAC7505.0931703E-1014.4980223
TACACTC7053.3087417E-914.395725
TACCCCG3050.00525209814.2608655
GTAGGAC5104.5007146E-614.2137483
TACACCG3700.001189359813.7148415
TAGTAGT7457.195922E-913.6223044
Produced by FastQC (version 0.11.5)