FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005718584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005718584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52696205
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG175550.060.430531
TGACTAG199000.051.26741
CGAGTAA99750.049.3176541
CGTGTAT119900.047.5288161
GATCTAG194200.045.715411
TATGTAG201500.043.869411
GATCTAA177600.043.527831
ATACTAA237100.042.9283371
ATACTTG304800.042.8200951
TATGTCG74100.042.1948551
CTACTAG151850.040.897271
CAACTAG150850.040.4394571
CGTGTAA116000.040.3894581
AATGTCG96100.040.345711
GATCTAT139550.040.322421
GAACTAG208500.038.6820031
TGACTAA273100.038.6699831
ATACTCG124700.038.4917071
TACTAGG153250.037.8752
GAACTAA208200.037.681471
Produced by FastQC (version 0.11.5)